Fig 2: Simultaneous immunofluorescence staining of cathepsin Z and MRP2 in hepatocytes. Liver specimens from non-diseased subjects (NL) and early- or jaundice-stage PBC were simultaneously stained with antibodies to cathepsin Z and bile canalicular marker MRP2 (×20). Nuclei were stained with DAPI (bule). Cathepsin Z (CTSZ; green) was observed proximal to bile canalicular marker MRP2 (red) in hepatocytes of NL (upper), whereas it was observed in the cytoplasm of hepatocytes from patients with early- (middle) and jaundice-stage PBC (lower). Scale bar: 50 μm.

Fig 3: Simultaneous immunofluorescence staining of cathepsin Z and LAMP1 in hepatocytes. Liver specimens from non-diseased subjects (NL) and early- or jaundice-stage PBC were simultaneously stained with antibodies to cathepsin Z and lysosomal marker LAMP1 (×63). Nuclei were stained with DAPI (bule). Colocalization of cathepsin Z (CTSZ; green) with LAMP1 (red) was observed in hepatocytes of NL (upper) and early-stage PBC (middle), whereas colocalization was rare in hepatocytes of jaundice-stage PBC (lower). Scale bar: 25 μm.